High Content Screening Drug Discovery

In most pharmaceutical and biotechnology companies there is a need to always improve the quality of lead candidates.

High content screening drug discovery.

Read more optimising phenotypic screening single cell analysis versus 3d multicell analysis by dr richard m. High content screening hcs also known as high content analysis hca or cellomics is a method that is used in biological research and drug discovery to identify substances such as small molecules peptides or rnai that alter the phenotype of a cell in a desired manner. This demand resulted in the use of cell based screening as a method of choice in drug discovery 1 2 high content screening hcs is multiplexed functional cell based screening 3 4 5 6 hcs can be used in all aspects of drug discovery as an engine for driving. A decade ago there was talk that high throughput screening hts was a contributory factor in drug discovery s declining productivity and that it stifled creativity and innovation.

1 but the last ten years have certainly disproved those claims with hts playing a role in the discovery of many new drugs and moving from the preserve of large pharma to a method available to smaller companies. Discovery of a novel rock2 inhibitor with anti migration effects via docking and high content drug screening 1 january 2016 molecular biosystems vol. 9 high content screening identifies inhibitors of the nuclear translocation of atf6. These types of assays are often referred to as high content screening or high content analysis hcs hca.

Paper phenotypic drug discovery with high content screening target based or phenotypic screening. The driving force to adopt imaging for drug development is the quantity and quality of cellular information that can be collected and the enhanced physiological relevance of cellular screening compared to biochemical screening. Recent developments in high content screening hcs technologies make it an attractive alternative for anti angiogenic drug discovery. Hence high content screening is a type of phenotypic screen conducted in cells involving the analysis of whole cells or.

In this study we present a novel high content screening assay which is highly predictive for human drug induced liver injury dili. Hcs integrates high throughput methodologies with automated multicolor fluorescence microscopy to collect quantitative morphological and molecular data from complex biological systems.